Gsdf Y created rich XX men inside the O. luzonensis
Phrase out-of Gsdf. (A) Real-date PCR away from Gsdf into the XX and you can XY fry away from dos months in advance of hatching (dbh) to help you 10 days immediately after hatching (dah). Bluish pubs, term out-of PG5 to the Y chromosome (Gsdf Y ); purple, the X chromosome (Gsdf X ). Significant differences were analyzed by using a few-method investigation out-of difference (ANOVA) accompanied by Bonferroni blog post-evaluating. Articles and error pubs portray suggest ± SEM (letter = 4 for every single developmental phase). (b–d) Into the situ hybridization away from Gsdf on gonad. (B) Phrase from Gsdf in the 5-dah XY fry. Gsdf was recognized simply inside somatic structure close germ tissue. (C) Expression out-of Gsdf in 5-dah XX fry. Weakened signals was basically observed. (D) Gsdf term in mature testis. Solid indicators had been seen for the Sertoli muscle (arrowheads) encompassing spermatogonia. Smaller signals was in fact found inside the efferent duct (ed). (E) Gsdf term within the mature ovary. Indicators was perceived regarding granulosa tissues (arrows) nearby really-put up oocytes (oc). nd, nephric duct; go, gonad; gu, instinct.
At 5 dah from the development gonads, support cells surrounding the newest germ tissue shown Gsdf both in XY and you can XX embryos, even though Gsdf phrase is actually a lot higher within the XY embryos ( Shape 3, B and C ). ادامه مطلب